Dissolve each vial of freeze-dried product with 10.5 ml of solvent.
Use aseptic technique during reconstitution and when removing aliquots from the vial. Adequately clean and disinfect the vial closure prior to each entry with a sterile needle.
Mix gently during reconstitution.
PLUSET is to be given by intramuscular injection only.
The following treatment schedule is recommended for the induction of superovulation in the cow:
The total recommended dose is 800 to 1000 IU in decreasing doses for 4 to 5 days. Considering the variability between animals and taking into account breed, age and reproductive status the dosing schedule should be adjusted appropriately. It is therefore recommended to seek veterinary advice on appropriate dosing patterns. For heifers and beef cows a total dose of 800 IU is recommended. For dairy cows the dose could be increased to 1000 IU taking into account increasing age, parity number and dairy production.
Recommended schedule for 800 IU in 4 days:
Day 1*
08:00 hrs
3.0 ml i.m.
(150 IU FSH + 150 IU LH)
20:00 hrs
3.0 ml i.m.
(150 IU FSH + 150 IU LH)
Day 2
08:00 hrs
2.5 ml i.m.
(125 IU FSH + 125 IU LH)
20:00 hrs
2.5 ml i.m.
(125 IU FSH + 125 IU LH)
Day 3**
08:00 hrs
1.5 ml i.m.
(75 IU FSH + 75 IU LH)
20:00 hrs
1.5 ml i.m
(75 IU FSH + 75 IU LH)
Day 4
08:00 hrs
1.0 ml i.m.
(50 IU FSH + 50 IU LH)
20:00 hrs
1.0 ml i.m.
(50 IU FSH + 50 IU LH)
Recommended schedule for 1000 IU in 5 days:
Day 1*
08:00 hrs
3.0 ml i.m.
(150 IU FSH + 150 IU LH)
20:00 hrs
3.0 ml i.m.
(150 IU FSH + 150 IU LH)
Day 2
08:00 hrs
2.5 ml i.m.
(125 IU FSH + 125 IU LH)
20:00 hrs
2.5 ml i.m.
(125 IU FSH + 125 IU LH)
Day 3**
08:00 hrs
2.0 ml i.m.
(100 IU FSH + 100 IU LH)
20:00 hrs
2.0 ml i.m
(100 IU FSH + 100 IU LH)
Day 4
08:00 hrs
1.5 ml i.m.
(75 IU FSH + 75 IU LH)
20:00 hrs
1.5 ml i.m.
(75 IU FSH + 75 IU LH)
Day 5
08:00 hrs
1.0 ml i.m.
(50 IU FSH + 50 IU LH)
20:00 hrs
1.0 ml i.m.
(50 IU FSH + 50 IU LH)
* Corresponds to the 11th day of the oestrus cycle.
** A luteolytic dose of prostaglandin F2 alpha should be administered intramuscularly at 60 and/or 72 hours after the beginning of superovulation treatment.