Preparation of Technetium Tc 99m-Sulfur Colloid:
Collect components: 10 ml. Reaction Vial, Solution A, Solution B, Lead Pig, Boiling Water Bath.
Draw up 1 ml. Solution A. (HCl solution) in syringe.
Draw up 2.5 ml. Tc-99m Pertechnetate containing desired activity, dilute with sterile normal saline if necessary.
Add the 2.5 ml Tc-99m Pertechnetate to the shielded reaction vial.
Immediately add the 1 ml. Solution A.
Vent vial to release excess vacuum. Remove vent before boiling.
Shake gently to mix.
Place shielded vial in boiling water bath for 5 minutes for liver imaging and 3 minutes for lymphoscintigraphy imaging use.
Remove from boiling water and let cool for a few minutes.
Draw up 1 ml. Solution B. (Phosphate Buffer) while reaction vial is cooling
After cooling Reaction vial, add the 1 ml Solution B and shake gently to mix. Caution: be sure vial is cool to avoid excess pressure
Quality control: ITLC-SG in normal saline. Greater than 95% should stay at the origin
Tc 99m Sulfur Colloid expires 6 hours after mixing
